Fig 1: Transcriptome-wide RNA sequencing assays in PCa DU145 cells.a Transcriptome strategy of RNA-sequencing conducted on DU145 cells exposed to YK-2-69 (3 µM) for 48 h. The shNC, shDYRK2, DMSO, and YK-2-69 groups all contain two biological replicates. Venn diagram of upregulated and downregulated signaling pathways in DYRK2 KD- and YK-2-69-treated DU145 cells. The number of genes in every signaling pathway is >50. Normalized enrichment score (NES) >1 or <-1; P < 0.05; FDR < 0.25. b The signaling pathways enriched in different groups obtained through Gene Set Enrichment Analysis (GSEA). c The core-enriched decreased (blue) and increased (red) signaling pathways in shDYRK2 and YK-2-69 treatment groups when compared with shNC and DMSO groups, respectively. The signaling pathways with P < 0.05 are presented. d The relative abundance of genes involved in MYC target V1, MYC target V2 and Mitotic SPINDLE in DYRK2 KD- and YK-2-69-treated DU145 cells. n = 2. The whiskers of boxplot represent the quantile percentile, from bottom to top are minima, 25%, median, 75%, and maxima respectively. Two-tailed Student’s t test was applied without adjustment for multiple comparisons (FDR). e GSEA was used to analyze the effects of DYRK2 KD or YK-2-69 treatment on the ANDROGEN RESPONSE signaling pathway in DU145 cells. f Volcano plot of significantly affected genes (absolute fold change > 2, P < 0.05) in DU-145 shDYRK2 group relative to shNC group and YK-2-69 group relative to DMSO group. The negative binomial distribution test of DESeq2 software was used. g Effects of DYRK2 KD or YK-2-69 treatment on the RRS1, GRWD1, CCNG2, and YPEL3 mRNA levels in DU145 cells. Unpaired two-tailed Student’s t test. Error bar, mean ± SD, n = 3. h The principal component analysis was used to identify transcriptome differences between two samples. i The different signaling pathways enriched in DYRK2 KD and YK-2-69 treatment groups obtained through GSEA. Source data are provided as a Source Data file.
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